Alan Saluk
Senior Scientific Director
(858) 784-8251
Flow Cytometry
The Flow Cytometry Core Facility is home to a variety of fluorescence-based analytical and sorting cytometers, as well as a skilled staff who can offer services, training, insight, and support.
Research applications of flow cytometry include:
- Multi-color Immunophenotyping
- High-Speed Cell Sorting
- Plate-based Screening
- Functional Cellular Assays
- Assay Design Support
- Introductory Flow Training
Please read below to learn more about the services offered.
Flow Cytometry at Scripps Research
Instruments
Astrios A & B | Beckman Coulter | Jet-in-Air Sorter
MoFlo Astrios EQ jet-in-air sorting flow cytometer
Astrios (A)
| Model: | MoFlo Astrios EQ |
| Software: | Summit 6.3 |
| Function: | Cell Sorter |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | 3 detectors for a 355nm Ultraviolet laser 4 detectors for a 405nm Violet laser 9 detectors for a 488nm Blue laser 5 detectors for a 561nm Green-Yellow laser 4 detectors for a 640nm Red laser |
| Detector Layout: |  |
| Nozzles: | 70 µm - Up to 72 million cells per hour 100 µm - Up to 36 million cells per hour |
| Sorting: | 1 - 6 sort streams 1.5 ml, 5 ml, 15 ml, 50 ml tubes Multiwell plates (up to 1536-well) |
| FluoroFinder: | [Click here] |
| Brochure: | [Click here] |
Astrios (B)
| Model: | MoFlo Astrios EQ |
| Software: | Summit 6.3 |
| Function: | Cell Sorter |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | 3 detectors for a 405nm Violet laser 9 detectors for a 488nm Blue laser 4 detectors for a 561nm Green-Yellow laser 4 detectors for a 640nm Red laser |
| Detector Layout: |  |
| Nozzles: | 70 µm - Up to 72 million cells per hour 100 µm - Up to 36 million cells per hour |
| Sorting: | 1 - 6 sort streams 1.5 ml, 5 ml, 15 ml, 50 ml tubes Multiwell plates (up to 1536-well) |
| FluoroFinder: | [Click here] |
| Brochure: | [Click here] |
Aurora A, B, C, & D | Cytek Biosciences | Spectral Analyzer
Aurora spectral analytical flow cytometer
Aurora A & B
| Model: | Aurora |
| Software: | SpectroFlo |
| Function: | Spectral Analyzer |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: |
Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor array per laser enabling more efficient spectrum capture in the 365-829 nm range. No filter changes required for any fluorochrome excited by the 355 nm, 405 nm, 488 nm, 561 nm, 640 nm lasers. |
| Detector Layout: |
|
| Brochure: | [Click here] |
Bigfoot | Invitrogen | Spectral Sorter
Propel Bigfoot
| Model: | Bigfoot |
| Software: | Sasquatch Software (SQS) |
| Function: | Cell Sorting (Spectral and conventional) |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | Excitation Lasers 349nm 405nm 445nm 488nm 532nm 561nm 594nm 640nm 785nm Detectors 60 parameters including FSC and SSC |
| Dector Layout: |
349nm – 12 Parameters 405nm – 12 Parameter 488nm/532nm – 7 Parameters 561nm/594 – 12 Parameters 640nm – 4 Parameters 445nm – 4 Parameters 785nm – 3 Parameters 55 Total Fluorescent Parameters |
| Nozzles: |
50, 70, 100, 120, and 150µm ceramic nozzle tips with adjustable pressure settings |
| Sorting: |
Up to 6-way sorting into tubes; configurable tube holders include 1.5mL, 5mL, 15mL, and 50mL adapters. Multi-way micro-well plate sorting up to 1536 wells. Temperature control (4-37°C) for all media types. |
| Brochure: | [Click here] |
NovoCyte A & B | ACEA Biosciences | Analyzer
NovoCyte A & B
| Model: | NovoCyte 3000 with NovoSampler Pro |
| Software: | BD FACS Diva 6 |
| Function: | Analyzer |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | 6 detectors for a 405nm Violet laser 7 detectors for a 488nm Blue laser 2 detectors for a 640nm Red laser |
| Dector Layout: |  |
| FluoroFinder: | [Click here] |
| Brochure: | [Click here] |
NovoCyte Quanteon A / Agilent / Analyzer
NovoCyte Quanteon A / Agilent / Analyzer
| Model: | Quanteon 4025 with NovoSampler Q |
| Software: | NovoExpress |
| Function: | Analyzer |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | Excitation Lasers: 488nm, 640nm, 405nm, 561nm Detection Parameters: 25 fluorescence + 2 scatter |
| Detector Layout: |  |
| Quanteon: | [Click here] |
Sony MA900 A & B | Sony BioSciences | Chip Cuvette Sorter
Sony MA900 (A & B)
| Model: | MA900 | |
| Software: | MA900 Software | |
| Function: | Cell Sorter |
|
| Location: | Immunology & Microbial Science, Room R12 | |
| Optics: | Excitation Lasers: 488 nm, 638 nm, 405 nm, 561 nm Detection Parameters:12 fluorescence + 2 scatter |
|
| Detector Layout: |  |
|
| Nozzles: |
70 μm - Up to 50 million cells per hour 100 μm - Up to 30 million cells per hour |
|
| Sorting: |
1-4 Sort Streams 0.5-mL, 1.5-mL, 5-mL and 15-mL tubes |
|
| FluoroFinder: | [Click here] | |
| Brochure: | [Click here] |
ZE5 (Yeti) A & B | Bio-Rad Laboratories | Analyzer
Yeti (A)
| Model: | ZE5 |
| Software: | Everest |
| Function: | Analyzer |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | 5 detectors for a 355nm Ultraviolet laser 7 detectors for a 405nm Violet laser 5 detectors for a 488nm Blue laser 7 detectors for a 561nm Green-Yellow laser 4 detectors for a 640nm Red laser |
| Dector Layout: |  |
| Brochure: | [Click here] |
Yeti (B)
| Model: | ZE5 |
| Software: | Everest |
| Function: | Analyzer |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | 7 detectors for a 405nm Violet laser 5 detectors for a 488nm Blue laser 7 detectors for a 561nm Green-Yellow laser 4 detectors for a 640nm Red laser |
| Dector Layout: |  |
| Brochure: | [Click here] |
FACSDiscover S8 | BD | Spectral & Imaging Sorter
FACSDiscover S8 Cell Sorter
| Model: | FACSDiscover S8 Cell Sorter |
| Software: | FACSChorus |
| Function: | Cell Sorting (Spectral and imaging) |
| Location: | Immunology & Microbial Science, Room R12 |
| Optics: | Excitation Lasers 349nm 405nm 488nm 561nm 637nm Detectors 78 fluorscence APD detectors |
| Detector Layout: | Fluorescence: 349 nm - 22 UV detectors, 365-860nm 405 nm - 20 Violet detectors, 410-860nm 488 nm - 16 Blue detectors, 495-860 nm 561m - 12 Yellow-green detectors, 570-860nm 637nm - 8 Red detectors, 645-860nm Imaging 488nm: FL1: LP505: 534/46 FL2: LP570: 600/60 FL3: LP675: 788/225 |
| Nozzles: | 85µm, 35psi, 57KHz 100µm, 20psi, 35KHz 130µm, 7psi, 15KHz |
| Sorting: | 85µm Up to 6-way sorting into 1.5 & 5ml tubes 100 & 130 2-way sorting in 1.5 & 5 ml tubes Plate sorting up to 384 well plates |
| Brochure: | [Click here] |
Reservations
Analyzer Calendar
Click here to login to Inifinity Calendar
Reservations for Analytical Cyometers are made using the IdeaElan, Infinity app. The app can be accessed through the TSRI My Apps/Single Sign-on or through the link above.
Sorting Reservations
Sorting reservations are made by email. You must email flowstaff@scripps.edu.
It is highly recommended that you plan ahead for your sorts. If you need a specific instrument on a specific day, place reservations two weeks ahead. If you're simply doing a single-color sort, you may be able to make the reservation in less time, but try to place your reservation at least a week in advance. Appointment availability varies throughout the year.
Do not start any upstream processes (i.e. injections, transfections, thaws, stimulations) unless you have a reservation already made.
Please include the following information for all sort requests:
- Name
- Scripps Research Account Code
- Principle Investigator
- Organization
- PO Number (if not affiliated with Scripps Research)
- Desired Sort Dates/Times
- Cell Origin/Species
- Tissue or cell line name
- Parameters (include all fluorescent antibodies, proteins, and dyes)
- Number of Samples to sort
- Estimated number of cells per sample
- Collection methood (description of tubes or plates)
- Purpose of sort (culture, DNA/RNA isolation, transplantation, etc.)
Resources
Getting Started
-
- Request Access to Flow Cytometery
- [Internal Scripps User Access Request]
- External User Access
- Contact flowstaff@scripps.edu
- Request Access to Flow Cytometery
Protocols & Procedures
- [Analyzer Cleanup] - All users are responsible for properly cleaning and/or shutting down the analyzers when they finish. Click the link for details.
- [Fluorochrome Index]
- [Sort Buffer Recipe] - Use this recipe as a starting point for your sorting buffer.
- [Sort Optimization] - Here are recommendations for cell preparation for sorting.
- [Current Protocols in Cytometry] - Scripps Research Network or valid login only
- [Cytometry Protocols] - Purdue University Cytometry Laboratories
Fluorescence Spectra
- [Fluorescence Spectrum Viewer] - BD Biosciences
- [FluoroFinder] - Use this tool to develop fluorescent panels and source antibodies.
- [Fluorescence Spectra Analyzer] - BioLegend
- [FluorPlan] - eBioscience
- [Spectra Viewer] - Chroma Technology Corp
Training & E-Learning
- [Introduction to Flow Cytometry Web-Based Training] - BD Biosciences
- [Multicolor Flow Cytometry Resources] - BD Biosciences
- [Multicolor Flow Cytometry Tools] - BD Biosciences
- [Flow Cytometry Resource Center] - Life Technologies
- [Introduction to Cytometry I] - J. Paul Robinson, 2005
- [Introduction to Cytometry II] - J. Paul Robinson, 2005
- [Multicolor Flow Cytometry Webinars] - BD Biosciences
- [Flow Cytometry Webinars] - Molecular Probes
- [Cytometry Data Analysis Tutorials] - FlowJo
- [Flow Cytometry - A Basic Introduction] - Michael G Ormerod, 2008
- [Practical Flow Cytometry] - Howard M. Shapiro, 2003
Flow Cytometry Organizations
Instrument Manuals
Aurora
LSR
NovoCyte
Sony MA900
ZE5
LSR
- BD FACSDiva v6_LSR_Quick Ref Guide
- BD FACSDiva Software Basic Experiment Guide
- BD FACSDiva_Software_v_8.0_manual
- BD LSR II User Guide
NovoCyte
Sony MA900
ZE5
Events
2024 Events
Advances in Cell Sorting Symposium
Join us for a symposium and in-person networking as we explore current innovations and trends in spectral and image cytometry on Thursday, September 19, 2024 from 9:00 a.m. to 5:00 p.m.
SPEAKERS
Nate Trujillo Thermo Fisher Scientific Title: Workflow Optimization for Flexible Sorting Applications on the Bigfoot Spectral Cell Sorter
David Vrane Cytek Biosciences “Advances in Next Gen Cell Sorting - Cytek Aurora CS”
Cedric Ait-Mansour Sony Biotechnology “FP7000 Spectral Cell Sorter – Usability and Performance for High-Parameter Experiments”
Mirko Corselli Becton-Dickinson “BD FACSDiscover Cell Sorter”
Willem Westra ThinkCyte “VisionSort: Voyages in Sorting with Morphometric Profiling & AI”
Rea Dabelic – Head of Application Development NanoCellect “The Future of Image-Guided Cell Sorting”
SPONSORS
Sony Biotechnology BD Biosciences Cytek Biosciences Thermofisher Scientific
NanoCellect
HOST
Flow Cytometry Core Facility
LOCATION
Scripps Research Auditorium 10620 John Jay Hopkins Drive San Diego, CA 92121
REGISTER
To confirm your attendance, please click here to complete the registration form.
To attend virtually via Zoom, please use this link on the day of the event.
CONTACT
Al Saluk, Senior Scientific Director (asaluk@scripps.edu)
Brian Seegers, Sort Facility Manager (bseegers@scripps.edu)
(858) 784-8251
Lunch and Learn BD BioSciences S8 Cell Sorter, January 11, 2024
Please join us for a lunch and learn seminar presented by BD Biosciences.
Seminar: Imaging and spectral capabilities of the BD FACSDiscovers S8 Cell Sorter (S8 brochure attached)
Presented by: Mirko Corselli, PhD - Associate Director of Market Develop, BD Biosciences
Date: Thursday, January 11
Time: Talk 12-1:00 PM, Lunch starts at 11:45am
Location: IMM East Conference Room (IMM-204X)
RSVP: Google Form
Contact: flowstaff@scripps.edu with any questions
2023 Events
Lunch & Learn BioLegend, December 12, 2023
Please join us for a lunch and learn seminar presented by BioLegend.
Seminar: Flow Panel Design and the latest BioLegend updates
Presented by: Nadja Tessler, M.Sc., BioLegend Technical Application Scientist,
Date: Tuesday, December 12, 2023
Time: 12-1:00 PM
Location: Imm East Conference Room (IMM-204X)
RSVP: nadja.tessler@biolegend.com
Lunch & Learn ThermoFisher, November 7, 2023
Please join us for a scientist-to-scientist lunch and learn seminar focusing on spectral cell sorting. Lunch provided by Thermo Fisher Scientific
Seminar: Employing panel design and control optimization best practices to analyze and sort elusive cells from high parameter panels (Flyer Attached)
Presented by: Luise Sternberg, PhD, Staff Scientist, R&D, Protein and Cell Analysis, Thermo Fisher Scientific
Date: Tuesday, November 7, 2023
Time: 12-1:00 PM
Location: IMM East Conference Room (IMM 204X)
RSVP: Email flowstaff@scripps.edu
Lunch & Learn: Cytek BioSciences
Please join us for a lunch and learn seminar focusing on the Cytek Cloud and other resources available to support Aurora users.
Seminar: Tips and Tricks from Cytek for using the Aurora
Presented by: Matt McBrian, Cytek Technical Sales Representative and Sarah Christy, Cytek Reagent Account Manager
Date: Tuesday, October 3, 2023
Time: 12-1:00 PM
Location: Tips and Tricks from Cytek for using the Aurora
RSVP: Register using link in flier or Click here. Any questions contact flowstaff@scripps.edu
Lunch & Learn: FluoroFinder
Please join us for a lunch and learn seminar to find out more about IntelliPanel from FluoroFinder.
Seminar: Tools for Panel Design and Spectral Visualization
Does it feel like you have to be an expert regarding reagents, instrumentation and biology to build an optimized multicolor flow cytometry panel? The complexity can feel overwhelming as the technology progresses past 18 fluorescent parameters and into >30 color Spectral Flow Cytometry. IntelliPanel from FluoroFinder is an application that automatically recommends reagent combinations based on the specific instrument configurations in your lab, antigen abundance, spectral spillover between fluorophores and overall spectral complexity of co-expression groups within the panel. IntelliPanel is also driven by machine learning, where experts make common changes and choices that inform future recommendations.
Presented by: Kelly Lundsten, Director Product Innovation, FluoroFinder
Date: Tuesday, August 15, 2023
Time: 12-1:00 PM
Location: Beckman Keck Amphitheater (Keck)
RSVP: (including any dietary restrictions): flowstaff@scripps.edu
Lunch & Learn BioRad
Please join us for a lunch and learn seminar to find out more about the Bio-Rad StarBright Dyes.
Seminar: Build Bigger, Better Panels with StarBrightTM Dyes from Bio-Rad
With the advancement of technology, flow cytometers are now able to detect a much greater range of fluorescent dyes, facilitating the analysis of more extensive and complex multicolor immunophenotyping assays. However, this has also introduced more pitfalls to navigate during experimental design.
Join this lunch and learn seminar to find out how StarBright Dyes and best practices can help circumvent many of these issues, enabling consistent and reliable results.
Presented by: Anjilna Wali, Bio-Rad
Date: Tuesday, August 1, 2023
Time: 12-1:45 PM
Location: Immunology East Conference Room (IMM-204X)
RSVP: (including any dietary restrictions): flowstaff@scripps.edu
2022 Events
Spectral Cytometry Symposium: Thursday, October 6, 2022
Join us for a symposium and in-person networking as we explore current innovations and trends in spectral cytometry on Thursday, October 6, 2022 from 9:00 a.m. to 5:00 p.m.
SPEAKERS
Thiago Alves da Costa, PhD
Thermo Fisher Scientific
Title: Novel tools and practical considerations for identifying and sorting elusive cells with spectral cytometry
Maria Jaimes, PhD
Cytek Biosciences
Title: The road to a high resolution 40-color flow cytometry immunophenotyping panel
Cedric Ait-Mansour, PhD
Sony Biotechnology
Title: ID7000 – Delivering intuitive tools for cell analysis of high dimensional immune profiling applications
Geoff Kraker, PhD
OMIQ
Title: A more efficient path to results: Overcoming barriers to data analysis with OMIQ
Kamila Czechowska, PhD
METAFlow by Metafora
Title: An innovative topological analysis platform for High-Dimensional Flow Cytometry Data
SPONSORS
Sony Biotechnology
BD Biosciences
Cytek Biosciences
Thermofisher Scientific
HOST
Flow Cytometry Core Facility
LOCATION
Scripps Research Auditorium
10620 John Jay Hopkins Drive
San Diego, CA 92121
REGISTER
To confirm your attendance, please click here to complete the registration form.
To attend virtually via Zoom, please use this link on the day of the event.
In order to attend, we do require proof of vaccination or a negative COVID-19 test result taken within 48 hours of the event. This information will be collected on the day of the event at registration. Masks are not required but may be worn based on personal preference. COVID-19 requirements are subject to change based on campus policy.
CONTACT
Al Saluk, Senior Scientific Director
(858) 784-8251
Pricing
Current rates per hour
|
Scripps Research |
Academic |
Pharma |
|
|
Analyzers |
$70 |
$180 |
$250 |
|
Sorting |
$95 |
$250 |
$300 |
Contact/Staff
FLOW CORE STAFF
Our core facility staff is happy to help you with any questions or concerns you may have. Please direct all inquiries about facility instruments or services to our main email address, flowstaff@scripps.edu or our main phone number, (858) 784-8396. Our email address will reach all of our staff members, so that any one of us may help you as quickly as possible.
Physical Address
Scripps Research
IMS Building, Floor 1, Room R12
10466 North Torrey Pines Road
La Jolla, CA 92037
(858) 784-8396
Mailing Address
Scripps Research
Flow Cytometry, IMM-20
104550 North Torrey Pines Road
La Jolla, CA 92037
104550 North Torrey Pines Road
La Jolla, CA 92037
Shipping Address
Scripps Research
9060 Activity Road, Unit F
San Diego, CA 92126
9060 Activity Road, Unit F
San Diego, CA 92126
ACCESSING OUR LAB
Visitor parking is located in Lot 4 (toward the center of the map). During normal business hours a parking permit is required. Obtain a permit from a Scripps Research security staff-member stationed at the entrance to that parking lot and park in an available space. Do not enter the hospital patient parking lot or you will be required to pay for parking.
Enter the Immunology & Microbial Science Building through the breezeway to the south (left on the map) of Lot 4. If the door is locked, use the call box located to the left of the front entrance. The call box allows us to buzz you in. Sign in at the front desk and take the elevator in the northwest corner (top-right of the building according to the map) to the first floor. The elevator near the front desk does not reach our facility. On the 1st floor you will find the Flow Cytometry Core Facility in room R12.
Click here for campus map